Infectious abortion is a significant cause ofreproductive failure and economic losses in cattle. The goal ofthis study was to detect nucleic acids of several infectious agentsknown to cause abortion including Arcanobacterium pyogenes,Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetussubsp. venerealis, Chlamydophila abortus, Leptospira sp.,Listeria monocytogenes, Salmonella sp., Mycoplasma bovis,Mycoplasma bovigenitalium, Neospora caninum, andTritrichomonas foetus. Tissue homogenates from 42 fetusesand paraffin-embedded tissues from 28 fetuses and 14placentas/endometrium were included in this study. Brucellaabortus was detected in 14.2% (12/84) of the samples.Salmonella sp. DNA was amplified from 2 fetuses, and therewas one positive for Neospora caninum, and another for Listeriamonocytogenes. This PCR-based approach resulted inidentification of the etiology in 19% of samples, or 20% ifconsidered fetal tissues only.
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